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To evaluate this, amino acid permeability of the perialgal vacuole (pv) membrane, which is a barrier between the host and its symbiotic chlorella, was investigated. Ten amino acids (arg, lys, his, phe, trp, tyr, gln, asn, ile and leu) are actively transported into vacuole, whereas acidic amino acids (glu and asp) are exclusively localized in cytosol. Since japanese symbionts preferred several amino acids for their growth, amino acid uptake were studied
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Localization of perialgal vacuoles beneath the host cell surface is not a prerequisite phenomenon for protection from the host's lysosomal fusion in the ciliate paramecium bursaria Of the ciliate paramecium bursaria To analyze the amino acid transport systems, the uptake of all amino acids was performed using radioactive tracer methods by kinetic analyses (kato and imamura 2009).